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The location and functional properties of antigen-specific memory T-cell populations in lymphoid and nonlymphoid compartments following DNA immunization or infection with Salmonella were investigated. Epitope-specific CD8+-T-cell expansion and retention during the memory phase were analyzed for DNA-immunized mice by use of a 5-h peptide restimulation assay. These data revealed that epitope-specifi
This paper investigates how vascular plants affect carbon flow and the formation and emission of the greenhouse gas methane (CH4 ) in an arctic wet tundra ecosystem in NE Greenland. We present a field experiment where we studied, in particular, how species-specific root exudation patterns affect the availability of acetate, a hypothesized precursor of CH4 formation. We found significantly higher a
The root/organic soil concentration ratio; R/S) of 50 cationic mineral elements was related to their ionic properties, including ionic radius (r), ionic charge (z), and ionic potential (z/r or z(2)/r). The materials studied were ectomycorrhizal beech (Fagus sylvatica L.) roots and their almost purely organic soil substrate, the O-horizon (mor; raw humus) of a Podzol in South Sweden, developed in a
A lysimeter method was evaluated for its suitability in gas emission studies by studying the effect of temperature on CO2 emissions (dark respiration) from cultivated peat soils. The study was carried out with organic soils from two locations in Sweden, a typical cultivated fen peat with low pH and high organic matter content (Orke) and a more uncommon fen peat with high pH and low organic matter
Waxing and waning ice sheets and changing sea levels have been interpreted from the Quaternary stratigraphic record at Leinstranda, Broggerhalvoya in NW Svalbard. We have identified seven high relative sea-level events, related to glacio-isostatic loading, and separated by at least four glacial events. To establish a chronology for the high sea-level events (interstadials and interglacials) and th
Background. Methylmalonic acid (MMA), a sensitive biomarker of functional vitamin B12 deficiency, is commonly determined by gas chromatography-mass spectrometry (GC-MS) or liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods using manual extraction and derivatization of MMA to reduce polarity prior to separation. Methods. In the present study we introduce a semi-automated extraction o
Galectins are carbohydrate binding proteins, implicated in conditions of both inflammation and cancer. Connections between chronic inflammation and cancer are proposed by the increased remodelling and proliferation that occurs, leading to enhanced survival and proliferation of malignant cells. Since galectins have been implicated in mechanisms of both chronic inflammation and cancer, we have inves
High-elevation grasslands of the Cyperaceae Kobresia pygmaea cover nearly half a million km2 on the Tibetan Plateau. As a consequence of climate change, precipitation patterns in this monsooninfluenced region may change with possible consequences for grassland productivity. Yet, not much is known about the water cycle in this second largest alpine ecosystem of the world. We measured the evapotrans
The A4GALT locus encodes a glycosyltransferase that synthesizes the terminal Galα1-4Gal of the P(k)(Gb3/CD77) glycosphingolipid, important in transfusion medicine, obstetrics and pathogen susceptibility. Critical nucleotide changes in A4GALT not only abolish P(k) formation but also another Galα1-4Gal-defined antigen, P1, which belongs to the only blood group system for which the responsible locus
We analysed the responses of 11 ecosystem models to elevated atmospheric [CO2] (eCO(2)) at two temperate forest ecosystems (Duke and Oak Ridge National Laboratory (ORNL) Free-Air CO2 Enrichment (FACE) experiments) to test alternative representations of carbon (C)-nitrogen (N) cycle processes. We decomposed the model responses into component processes affecting the response to eCO(2) and confronted
This study increases the understanding at a molecular level of the interactions between DNA and poly(amido amine) (PAMAM) dendrimers on solid surfaces, which is a subject of potential interest in applications such as gene therapy. We have used in situ null ellipsometry and neutron reflectometry to study the structure of multilayer arrangements formed by PAMAM dendrimers of generation 2 (G2), 4 (G4
Pheromones are central to the mating systems of a wide range of organisms, and reproductive isolation between closely related species is often achieved by subtle differences in pheromone composition. In insects and moths in particular, the use of structurally similar components in different blend ratios is usually sufficient to impede gene flow between taxa. To date, the genetic changes associated
Summary. Nonacog alfa, a recombinant factor IX (FIX) used for the treatment of haemophilia B, was approved in Europe in 1998. In accordance with European Medicines Agency requirements, a registry study was conducted from 2002 to 2009. A reformulated iso-osmotic version was approved for European use in 2007. This study was conducted to evaluate the safety of nonacog alfa in a usual care setting, an
The Src-Like Adaptor Protein (SLAP) is an adaptor protein sharing considerable structural homology with Src. SLAP is expressed in variety of cells regulating receptor tyrosine kinase signaling by direct association. In this report, we show that SLAP associates with both wild-type and oncogenic c-Kit (c-Kit-D816V). The association involves SLAP SH2 domain and receptor phosphotyrosine residues diffe
Background: It has been found that patch testing with 15μl formaldehyde 2.0% aq detects twice more allergies than testing with 1.0%. The clinical relevance of positive patch test reactions is often difficult to determine. Use tests are simple to do and help to evaluate the significance of patch test results. Objectives: To study the clinical relevance of contact allergy to formaldehyde detected by
The role of nurses and technicians in the treatment of ST-elevation myocardial infarction (STEMI) by primary percutaneous coronary intervention (PPCI) is vital for the success of the multidisciplinary Heart Team. Several editorials have emphasised the importance of a holistic treatment which links each step of care to the next, a chain that is essential for quality and efficacy in the management o
